Biotechnology

 

Universities Involved

University College Dublin
Queen's University Belfast
University of Ulster

Department(s)

Department of Crop Science Horticulture and Forestry
National Agricultural & Veterinary Biotechnology Centre

Project Title / Description

Centre for Innovation in Biotechnology (CIB) ­ IFI Mushroom Demonstration Project. Centre for innovation in Biotechnology 1994­1997

The main objective is, to assist the development of a Biotec infrastructure in Northern Ireland with the assistance of Bio Research Ireland.

Project funded: CIB c. £800,000 ­ IFI (mushroom project £382,000 ­ IFI£50,000 ­ mushroom industry R.O.I.)

Contacts

Dr Jeremy Carmichael
Tel 04890 335471

 

 

Business Involvement

Garry Graham, Irish Mushroom Growers Association Tel + 353 1 450 0266/455 1043

Development Potential

Yes. CIB submitted proposal for continued funding to IRTU in Northern Ireland.


Universities Involved

Dublin City University
Queen's University Belfast
University of Ulster, Coleraine

Department(s)

School of Biotechnology

Project Title / Description

Biosorption of metal and dyes.

The potential collaboration is in the initial and developmental stages. The objective is to further the knowledge of the subject. Open to new collaborators.

Collaboration began with exchange visits in 1998. This was followed by attendance and presentation of a poster paper by DCU researchers at the International Environmental Biotechnology Symposium held in QUB in summer 1998.

The second strand of the potential collaboration is with Dr Anthony McHale and Dr Geoff McMullin of UU Coleraine who are also involved in the study of biosorption systems.

Contacts

Dr Stephen Allen
Tel 04890 274523/381753

Dr Anthony McHale and Dr Geoff McMullin
Tel 04870 324616

Dr John Tobin
Tel + 353 1 7045408
John.tobin@dcu.ie

Development Potential

All three potential partners are interested in collaborative work and would welcome development of the project. As above this is a growing and promising research area. Funding is the main obstacle to further co­operation.


Universities Involved

Dublin City University
Queen's University Belfast

Department(s)

School of Biotechnology

Project Title / Description

Trypsin­catalysed peptide synthesis The objective of the project was to use the pancreatic digestive enzyme trypsin for synthesis of peptides and to compare the catalytic properties of chemically stabilised trypsin derivatives with those of the native protein.

Two refereed papers were produced:

  • 'Chemically stabilised trypsin used in di­peptide synthesis.' Biotechnology & Bioengineering 58 366­373.
  • Di­peptide synthesis using acelylated trypsin derivative' Biotechnology Techniques 11 13­16.

Contacts

Prof. Brian Walker
Brian.walker@qub.ac.uk

Prof. Martin Clynes
martin.clynes@dcu.ie

 

Business Involvement

Biosyn Ltd, 97 Lisburn Road, Belfast

Development Potential

All three potential partners are interested in collaborative work and would welcome development of the project. As above this is a growing and promising research area. Funding is the main obstacle to further co­operation.


Universities Involved

University College Cork
Queen's University Belfast

Department(s)

Microbiology Department
(BIOMERIT Research Centre)

Other Universities

University of Marburg, Germany
University of Helsinki, Finland
Leiden University, The Netherlands
Agricultural University of Athens, Greece
As well as the following research centres: CNRS ­France; CSIC­Spain; John Innes Centre­U.K.

Project Title / Description

Symbiosis and defence in the interaction of plants with micro­organism.

The objective of the project is to elucidate the molecular basis of competition, specificity and signalling in bacterial­legume symbiosis, to study the rhizobia infection process and triggering of defence reactions. In addition, membrane targeting, defence reactions and symbiosis specific gene expressions will be studied as
well as the biological control with rhizosphere bacteria and arbuscular mycorrhiza fungi.

The outcome it is hoped will show the following:
1. The identification of new genes responsible for competitiveness, host specificity and signalling.
2. The identification of new mechanisms of repression, of defence reactions, during the early stages of symbiosis development.
3. The identification of new mechanisms
of triggering and repression, of defence reactions in later stages of symbiosis development, such as nodule development.
4.Improvement of biological control agents including Pseudomonous and arbuscular mycirrhiza.

To date the following has been done:

  • Molecular characterisation of the secondary metabolite 2, 4­diacetylphoroglucinol (Phl) which is a major compound responsible for Pseudomonous fluorescens F113 isolated from the sugarbeet rhizophere, biological activity towards the pathogenic fungus Pythium ultimun.
  • The biosynthetic genes of Phl (phlACBD) were located, sequenced and identified in addition the regulatory loci phlF, gacA, gacS and rrl were elucidated.

Contacts

Prof. D Werner
Tel + 49 6421 281546 Fax + 49 6421 288997
Werner@mailer.uni­marburg.de

Prof. Fergal O'Gara, UCC
Tel + 353 21 902 646 Fax + 353 21 275934
F.ogara@ucc.ie

 

Development Potential

Research area being developed has a strong potential for further development in a multi- disciplinary framework and could fit in with the activities of Framework Five of the European Union.


Universities Involved

University College Cork
University of Ulster, Coleraine

Department(s)

Department of Microbiology

Project Title / Description

Combined biological and chemical remediation systems for the treatment of textile industry and related effluents.

The project aimed to design a reactor system for the remediation of organic and metal­ bearing wastewater from the textile industry. This would involve effective combination of chemical and biological treatments to provide enhanced remediation of effluents containing metals and persistent organics.

Future funding would ideally be for research scientists at the PhD level to work in both laboratories on both chemical and microbial based aspects of these systems.

Contacts

Dr Alan Dobson, UCC
Tel + 353 21 902743
Fax + 353 21 903101
a.dobson@ucc.ie

Dr Geoff McMullan, UU, Coleraine
Tel 04870 44141 ext 4755
Fax 04870 324906
g.mcmullan@ulst.ac.uk

 

Development Potential

Yes. Both laboratories are anxious to build upon the initial existing links through participation in a joint collaborative research project in the area of Environmental Biotechnology, which would be aimed at the exchange of expertise and personnel between the two laboratories, with the overall aim of developing biological and chemical based reactor systems for the remediation of problematic waste streams.


Universities Involved

University College Cork
Queen's University Belfast

Department(s)

Department of Microbiology

Project Title / Description

Physiology and Genetics of Micro­organisms involved in Biodegradative processes.

In 1993 an initial contact was made and ultimately resulted in a research scientist from Cork, spending a period of time at Queen's in the laboratory of Dr Larkin. This trip was funded through the EOLAS/British Council Scheme (Project no: 9346). This resulted in a joint grant application being made by both research groups in 1997­98 through North/South funding bodies.

The project aims to study the physiology and genetics of a number of different micro­ organisms and fungi which are capable of degrading a wide variety of environmental pollutants such as phenol, thiocyanate, nickel, styrene and polycyclic aromatic hydrocarbons.

Future funding would ideally be for research scientists at the PhD level to work in both laboratories on the physiology and genetics of the microbial systems involved in the biodegradation of a number of key environmental pollutants.

Contacts

Dr Alan Dobson, UCC
Tel + 353 21 902743
Fax + 353 21 903101
a.dobson@ucc.ie

Dr Mike Larkin, QUB
Tel 04890 245133 ext 2288
Fax 04890 236505
m.larkin@qub.ac.uk

 

Development Potential

A research scientist from Cork has previously spent a study period in Dr Larkin's laboratory at Queen's. Both laboratories are anxious to build upon the initial existing links through participation in a joint collaborative research project in the area of Environmental Biotechnology, which would be aimed at the exchange of expertise and personnel between the two laboratories, with the overall aim of the further physiological, biochemical and genetic analysis of the bacterial and fungal strains involved in degradative processes which are currently being studied in each of the two laboratories.


Universities Involved

University College Cork
Queen's University Belfast

Department(s)

Department of Microbiology
School of Biology, Questor Centre

Project Title / Description

Studies on Enhanced Biological Phosphate Removal from waste water treatment streams.

The project involves the use of novel microbial based approaches to the removal of phosphorous from wastewater streams. The basis of the project is the use of "Enhanced Biological Phosphate Removal" (EBPR) systems utilising micro­organisms/including yeasts which accumulate phosphates. The researchers at Queen's University are concentrating on the microbial physiology of the system and in the identification of the predominant species involved, while researchers in UCC are working on the genetics of the phosphate uptake systems in phosphate accumulating Acinetobacter strains. Work to date has resulted in the exchange of strains and protocols. It is hoped to expand the collaboration through the submission of a grant application to appropriate funding agencies in the near future. Funding required would be for research scientists at the PhD level to work in both laboratories on the physiology and genetics of the microbial systems involved in EBPR.

Contacts

Dr Alan Dobson, UCC
Tel + 353 21 902743
Fax + 353 21 903101
a.dobson@ucc.ie

Dr John P Quinn, QUB
Tel 04890 245133
Fax 04890 236505
j.quinn@qub.ac.uk

Dr John W McGrath, QUB
Tel 04890 245133 ext 2250
Fax 04890 236505
j.mcgrath@qub.ac.uk

Development Potential

Both laboratories are anxious to strengthen the existing links through participation in a joint collaborative research project which would be aimed at the exchange of expertise and personnel between the two laboratories, with the overall aim of the further physiological, biochemical and genetic analysis of the bacterial and yeast systems currently being studied in each of the two laboratories.


Universities Involved

University College Cork
Queen's University Belfast

Department(s)

Department of Plant Science

Project Title / Description

Genetic analysis of hatching activity towards potato cyst nematodes in potato.

The collaborative research has involved the UCC group analysing the inheritance of PCN hatching activity and hatching factor (HF) content in root leachate of inter­specific potato hybrids developed, as part of novel PCN control strategies by the QUB group.

The collaboration started in 1996 and is ongoing; contact between the two groups started when the UCC group co­ordinated an EU grant proposal to the FAIR programme, which included the QUB group. The hybrids were developed to develop a) PCN resistant potato varieties and b) a novel PCN trap crop; in both cases, high hatching activity would be desirable. In a) HFs for the two PCN species were shown to be species specific and the two classes of HF were inherited independently. Novel HFs (absent in both parents) were produced in some hybrids. Overall, HFs inherited from cultivated potatoes were more active than those from wild potatoes. Related work is due to start on b). The QUB group has also provided the UCC group with large amounts of PCN material for other aspects of PCN research. To date, the collaboration has been funded by grants to the individual research groups: Belfast (DANI/DARDNI) and Cork (BioResearch Ireland).
The main expenditure has been on research staff salaries. There have been three exchange visits. One paper has been submitted for publication ("Nematology") and a joint paper presented at the Association of Applied Biologists Meeting on Nematology in London, December 1999. Collaboration is open to
other groups.

Contacts

Dr Peter Jones, UCC
Tel + 353 21 902321/2324
p.jones@ucc.ie

Dr Colin Fleming
Tel 04890 25 5276
Fax 04890 66 8375
Colin.Fleming@dardni.gov.uk

Dr Susan Turner
Tel 04890 25 5280
Fax 04890 25 5380
Sue.Turner@dardni.gov.uk

Development Potential

For both groups, the objectives of their PCN research is to develop novel environmentally protective control measures against this pest. The complementary nature of the facilities and expertise of the two research groups means that collaboration increases the effectiveness of the research of each group. In the breeding work of the QUB group, analysis of HF content in UCC could accelerate development of high­ hatch resistant varieties or trap crops. In turn, the QUB group has developed PCN­infested sites for field trials; access to these would be
of tremendous benefit to the UCC group in optimising and trialing PCN control methods based on application of HFs to PCN­infested soil (inducing "suicide hatch"), either directly or via HF­producing rhizobacteria and mycorrhizal fungi.


Universities Involved

Dublin City University
Queen's University Belfast

Department(s)

School of Biotechnology

Project Title / Description

The role of peptidases in the regulation of neurotransmission signalling in the parasitic helminthes (worms) Fasciola hepatica and Schistosoma mansoni.

Schistosoma (blood flukes) and Fasciola (liver flukes) species of parasites have been studied extensively in the laboratories at QUB and DCU. While schistosomes are the most important worm disease with 200 million people infected, liver fluke disease in humans was recently recognised by the WHO as an important emerging pathogen of humans with at least 2.4 million people infected. Treatment against these parasites relies on chemotherapy. However, as a consequence of increasing drug resistance there is an urgent need to identify key parasite­specific biochemical pathways at which novel chemotherapies can be designed and targeted. In this context, the mechanism of neuromuscular co­ordination in parasites is important since this system is crucial to the survival of parasites and most front­line antiparasite chemotherapies presently used in some way interfere with parasite neuromuscular function. The aim of this project is to study the role of a novel aminopeptidase activity in the regulation of neuropeptide signal termination at the neuromuscular junction of these human parasites.

Both groups were familiar with each others' research and had expressed a desire to collaborate.

The collaboration could be extended to other interested in infectious diseases, drug design and vaccine.

It is hoped a) elucidate the mechanism by which this enzyme degrades neuropeptides and regulates neurotransmission in these worm parasite; b) show how the enzyme is synthesized and stored in the parasites' neurons; c) isolate the gene and express this in a eukaryotic system so that its biochemical and physiochemical properties can be studied and; d) synthesise inhibitors to demonstrate their ability to cause neuromuscular paralysis in the parasites.

Contacts

Dr Aaron Maule
Tel 04890 272059/236505 a.maule@qub.ac.uk

Prof. David Halton
Tel 04890 335792 Fax 04890 236505
d.halton@qub.ac.uk

 

Development Potential

Yes. Since both groups have a longstanding record in the field of Molecular Parasitology
it is intended to put together a joint research proposal to an international funding agency such as The Wellcome Trust.


Universities Involved

Queen’s University Belfast

Dublin City University

Department(s)

Department of Veterinary Sciences

Project Title / Description

(a) Multi-residue screening for antibiotic compounds in food by receptor and divergent antibody-based biosensor technology

 

(b) Screening of pesticides in foods

There has been ongoing collaboration/ discussions with Dr. Elliot on Food Analysis. Two proposals have been submitted to the EU 5th Framework. Exchange visits have been made. Protocols/materials have been exchanged. Funding has come from individual laboratory resources. The expertise/facilities of both groups are very complementary.

 

Contacts

Prof. Richard O’Kennedy, DCU

Tel + 353 1 700 5319

Fax + 353 1 700 5412

richard.okennedy@dcu.ie

Dr Chris Elliot, QUB

Tel 04890 525625

Fax 04890 761757

chris.elliot@dardmi.gov.uk

 

 


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